A medium-throughput crystallization approach
نویسندگان
چکیده
منابع مشابه
A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery
Objective(s): Here, a reporter cell line containing two reporter vectors were developed, in order to monitor the Human T-Lymphotropic Virus type1(HTLV-1) infectivity and the cell viability simultaneously. Materials and Methods: The reporter cell line was constructed by stably transfected baby hamster's kidney cell line (BHK-21), with the genomes expressing two different reporters in separate pl...
متن کاملHigh throughput pH optimization of protein crystallization.
Most high throughput structural proteomics centers use the sitting-drop method to obtain diffracting crystals for three-dimensional (3D) structure determination of biological macromolecules by x-ray crystallography. Although several robotic systems are available for dispensing the initial sitting-drop screening conditions, generally they are not used for optimization of crystallization conditio...
متن کاملDiscrete salt crystallization at the surface of a porous medium.
Efflorescence refers to crystallized salt structures that form at the surface of a porous medium. The challenge is to understand why these structures do not form everywhere at the surface of the porous medium but at some specific locations and why there exists an exclusion distance around an efflorescence where no new efflorescence forms. These are explained from a visualization experiment, por...
متن کاملA High - Throughput Screening Approach
The high-throughput search described in this paper takes advantage of multiple levels of parallelism, from coarse to fine-grained. Roughly speaking, fine-grained parallelism is exploited by allocating one core (of which modern graphics hardware have many; and their number is exponentially growing over the years) to one or more virtual neurons, while coarse-scale parallelism is achieved by alloc...
متن کاملThe open-access high-throughput crystallization facility at EMBL Hamburg.
Here, the establishment of Europe's largest high-throughput crystallization facility with open access to the general user community is reported. The facility covers every step in the crystallization process from the preparation of crystallization cocktails for initial or customized screens to the setup of hanging-drop vapour-diffusion experiments and their automatic imaging. In its first year o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Crystallographica Section D Biological Crystallography
سال: 2002
ISSN: 0907-4449
DOI: 10.1107/s0907444902013938